Bacterial Outer Membrane Vesicles (OMVs)-Based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV
Identifieur interne : 000685 ( Main/Exploration ); précédent : 000684; suivant : 000686Bacterial Outer Membrane Vesicles (OMVs)-Based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV
Auteurs : Mahmoud M. Shehata ; Ahmed Mostafa ; Lisa Teubner ; Sara H. Mahmoud ; Ahmed Kandeil ; Rabeh Elshesheny ; Thamer A. Boubak ; Renate Frantz ; Luigi La Pietra ; Stephan Pleschka ; Ahmed Osman ; Ghazi Kayali [Liban] ; Trinad Chakraborty ; Mohamed A. Ali ; Mobarak Abu MraheilSource :
- Vaccines [ 2076-393X ] ; 2019.
Abstract
Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290–650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.
Url:
DOI: 10.3390/vaccines7020046
PubMed: 31141982
PubMed Central: 6631769
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bacterial Outer Membrane Vesicles (OMVs)-Based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV</title><author><name sortKey="Shehata, Mahmoud M" sort="Shehata, Mahmoud M" uniqKey="Shehata M" first="Mahmoud M." last="Shehata">Mahmoud M. Shehata</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Mostafa, Ahmed" sort="Mostafa, Ahmed" uniqKey="Mostafa A" first="Ahmed" last="Mostafa">Ahmed Mostafa</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation><affiliation><nlm:aff id="af2-vaccines-07-00046">Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>Stephan.Pleschka@viro.med.uni-giessen.de</email></nlm:aff></affiliation></author><author><name sortKey="Teubner, Lisa" sort="Teubner, Lisa" uniqKey="Teubner L" first="Lisa" last="Teubner">Lisa Teubner</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Mahmoud, Sara H" sort="Mahmoud, Sara H" uniqKey="Mahmoud S" first="Sara H." last="Mahmoud">Sara H. Mahmoud</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Kandeil, Ahmed" sort="Kandeil, Ahmed" uniqKey="Kandeil A" first="Ahmed" last="Kandeil">Ahmed Kandeil</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Elshesheny, Rabeh" sort="Elshesheny, Rabeh" uniqKey="Elshesheny R" first="Rabeh" last="Elshesheny">Rabeh Elshesheny</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Boubak, Thamer A" sort="Boubak, Thamer A" uniqKey="Boubak T" first="Thamer A." last="Boubak">Thamer A. Boubak</name><affiliation><nlm:aff id="af4-vaccines-07-00046">Biological Department, Faculty of Science, King Abdul Aziz University, Jeddah 80203, Saudi Arabia;<email>Th_ah@hotmail.com</email></nlm:aff></affiliation></author><author><name sortKey="Frantz, Renate" sort="Frantz, Renate" uniqKey="Frantz R" first="Renate" last="Frantz">Renate Frantz</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Pietra, Luigi La" sort="Pietra, Luigi La" uniqKey="Pietra L" first="Luigi La" last="Pietra">Luigi La Pietra</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Pleschka, Stephan" sort="Pleschka, Stephan" uniqKey="Pleschka S" first="Stephan" last="Pleschka">Stephan Pleschka</name><affiliation><nlm:aff id="af2-vaccines-07-00046">Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>Stephan.Pleschka@viro.med.uni-giessen.de</email></nlm:aff></affiliation></author><author><name sortKey="Osman, Ahmed" sort="Osman, Ahmed" uniqKey="Osman A" first="Ahmed" last="Osman">Ahmed Osman</name><affiliation><nlm:aff id="af5-vaccines-07-00046">Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 38105, Egypt;<email>aoegiza@yahoo.com</email></nlm:aff></affiliation></author><author><name sortKey="Kayali, Ghazi" sort="Kayali, Ghazi" uniqKey="Kayali G" first="Ghazi" last="Kayali">Ghazi Kayali</name><affiliation><nlm:aff id="af6-vaccines-07-00046">Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030, USA;<email>ghazi@human-link.org</email></nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="af7-vaccines-07-00046">Human Link, Baabda 1109, Lebanon</nlm:aff><country xml:lang="fr">Liban</country><wicri:regionArea>Human Link, Baabda 1109</wicri:regionArea><wicri:noRegion>Baabda 1109</wicri:noRegion></affiliation></author><author><name sortKey="Chakraborty, Trinad" sort="Chakraborty, Trinad" uniqKey="Chakraborty T" first="Trinad" last="Chakraborty">Trinad Chakraborty</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Ali, Mohamed A" sort="Ali, Mohamed A" uniqKey="Ali M" first="Mohamed A." last="Ali">Mohamed A. Ali</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Mraheil, Mobarak Abu" sort="Mraheil, Mobarak Abu" uniqKey="Mraheil M" first="Mobarak Abu" last="Mraheil">Mobarak Abu Mraheil</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31141982</idno><idno type="pmc">6631769</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6631769</idno><idno type="RBID">PMC:6631769</idno><idno type="doi">10.3390/vaccines7020046</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">001177</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001177</idno><idno type="wicri:Area/Pmc/Curation">001177</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001177</idno><idno type="wicri:Area/Pmc/Checkpoint">000392</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000392</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:31141982</idno><idno type="wicri:Area/PubMed/Corpus">000519</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000519</idno><idno type="wicri:Area/PubMed/Curation">000519</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000519</idno><idno type="wicri:Area/PubMed/Checkpoint">000659</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000659</idno><idno type="wicri:Area/Ncbi/Merge">002238</idno><idno type="wicri:Area/Ncbi/Curation">002238</idno><idno type="wicri:Area/Ncbi/Checkpoint">002238</idno><idno type="wicri:Area/Main/Merge">000688</idno><idno type="wicri:Area/Main/Curation">000685</idno><idno type="wicri:Area/Main/Exploration">000685</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Bacterial Outer Membrane Vesicles (OMVs)-Based Dual Vaccine for Influenza A H1N1 Virus and MERS-CoV</title><author><name sortKey="Shehata, Mahmoud M" sort="Shehata, Mahmoud M" uniqKey="Shehata M" first="Mahmoud M." last="Shehata">Mahmoud M. Shehata</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Mostafa, Ahmed" sort="Mostafa, Ahmed" uniqKey="Mostafa A" first="Ahmed" last="Mostafa">Ahmed Mostafa</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation><affiliation><nlm:aff id="af2-vaccines-07-00046">Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>Stephan.Pleschka@viro.med.uni-giessen.de</email></nlm:aff></affiliation></author><author><name sortKey="Teubner, Lisa" sort="Teubner, Lisa" uniqKey="Teubner L" first="Lisa" last="Teubner">Lisa Teubner</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Mahmoud, Sara H" sort="Mahmoud, Sara H" uniqKey="Mahmoud S" first="Sara H." last="Mahmoud">Sara H. Mahmoud</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Kandeil, Ahmed" sort="Kandeil, Ahmed" uniqKey="Kandeil A" first="Ahmed" last="Kandeil">Ahmed Kandeil</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Elshesheny, Rabeh" sort="Elshesheny, Rabeh" uniqKey="Elshesheny R" first="Rabeh" last="Elshesheny">Rabeh Elshesheny</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Boubak, Thamer A" sort="Boubak, Thamer A" uniqKey="Boubak T" first="Thamer A." last="Boubak">Thamer A. Boubak</name><affiliation><nlm:aff id="af4-vaccines-07-00046">Biological Department, Faculty of Science, King Abdul Aziz University, Jeddah 80203, Saudi Arabia;<email>Th_ah@hotmail.com</email></nlm:aff></affiliation></author><author><name sortKey="Frantz, Renate" sort="Frantz, Renate" uniqKey="Frantz R" first="Renate" last="Frantz">Renate Frantz</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Pietra, Luigi La" sort="Pietra, Luigi La" uniqKey="Pietra L" first="Luigi La" last="Pietra">Luigi La Pietra</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Pleschka, Stephan" sort="Pleschka, Stephan" uniqKey="Pleschka S" first="Stephan" last="Pleschka">Stephan Pleschka</name><affiliation><nlm:aff id="af2-vaccines-07-00046">Institute of Medical Virology, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>Stephan.Pleschka@viro.med.uni-giessen.de</email></nlm:aff></affiliation></author><author><name sortKey="Osman, Ahmed" sort="Osman, Ahmed" uniqKey="Osman A" first="Ahmed" last="Osman">Ahmed Osman</name><affiliation><nlm:aff id="af5-vaccines-07-00046">Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo 38105, Egypt;<email>aoegiza@yahoo.com</email></nlm:aff></affiliation></author><author><name sortKey="Kayali, Ghazi" sort="Kayali, Ghazi" uniqKey="Kayali G" first="Ghazi" last="Kayali">Ghazi Kayali</name><affiliation><nlm:aff id="af6-vaccines-07-00046">Department of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas, Houston, TX 77030, USA;<email>ghazi@human-link.org</email></nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="af7-vaccines-07-00046">Human Link, Baabda 1109, Lebanon</nlm:aff><country xml:lang="fr">Liban</country><wicri:regionArea>Human Link, Baabda 1109</wicri:regionArea><wicri:noRegion>Baabda 1109</wicri:noRegion></affiliation></author><author><name sortKey="Chakraborty, Trinad" sort="Chakraborty, Trinad" uniqKey="Chakraborty T" first="Trinad" last="Chakraborty">Trinad Chakraborty</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author><author><name sortKey="Ali, Mohamed A" sort="Ali, Mohamed A" uniqKey="Ali M" first="Mohamed A." last="Ali">Mohamed A. Ali</name><affiliation><nlm:aff id="af1-vaccines-07-00046">Center of Scientific Excellence for Influenza Viruses, Environmental Research Division, National Research Centre (NRC), Cairo 12622, Egypt;<email>Mahmoud.Shehata@human-link.org</email> (M.M.S.);<email>ahmed_elsayed@daad-alumni.de</email> (A.M.);<email>sara.Hussein@human-link.org</email> (S.H.M.);<email>ahmed.Kandeil@human-link.org</email> (A.K.);<email>rabeh.elshesheny@stjude.org</email> (R.E.)</nlm:aff></affiliation></author><author><name sortKey="Mraheil, Mobarak Abu" sort="Mraheil, Mobarak Abu" uniqKey="Mraheil M" first="Mobarak Abu" last="Mraheil">Mobarak Abu Mraheil</name><affiliation><nlm:aff id="af3-vaccines-07-00046">Institute of Medical Microbiology, German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen Site, Justus-Liebig University Giessen, 35392 Giessen, Germany;<email>lisa.teubner@mikrobio.med.uni-giessen.de</email> (L.T.);<email>renate.frantz@mikrobio.med.uni-giessen.de</email> (R.F.);<email>luigi.La-pietra@mikrobio.med.uni-giessen.de</email> (L.L.P.);<email>trinad.chakraborty@mikrobio.med.uni-giessen.de</email> (T.C.)</nlm:aff></affiliation></author></analytic><series><title level="j">Vaccines</title><idno type="eISSN">2076-393X</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Vaccination is the most functional medical intervention to prophylactically control severe diseases caused by human-to-human or animal-to-human transmissible viral pathogens. Annually, seasonal influenza epidemics attack human populations leading to 290–650 thousand deaths/year worldwide. Recently, a novel Middle East Respiratory Syndrome Coronavirus emerged. Together, those two viruses present a significant public health burden in areas where they circulate. Herein, we generated a bacterial outer membrane vesicles (OMVs)-based vaccine presenting the antigenic stable chimeric fusion protein of the H1-type haemagglutinin (HA) of the pandemic influenza A virus (H1N1) strain from 2009 (H1N1pdm09) and the receptor binding domain (RBD) of the Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (OMVs-H1/RBD). Our results showed that the chimeric antigen could induce specific neutralizing antibodies against both strains leading to protection of immunized mice against H1N1pdm09 and efficient neutralization of MERS-CoV. This study demonstrate that OMVs-based vaccines presenting viral antigens provide a safe and reliable approach to protect against two different viral infections.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Bhuyan, G S" uniqKey="Bhuyan G">G.S. Bhuyan</name></author><author><name sortKey="Hossain, M A" uniqKey="Hossain M">M.A. Hossain</name></author><author><name sortKey="Sarker, S K" uniqKey="Sarker S">S.K. Sarker</name></author><author><name sortKey="Rahat, A" uniqKey="Rahat A">A. Rahat</name></author><author><name sortKey="Islam, M T" uniqKey="Islam M">M.T. Islam</name></author><author><name sortKey="Haque, T N" uniqKey="Haque T">T.N. Haque</name></author><author><name sortKey="Begum, N" uniqKey="Begum N">N. Begum</name></author><author><name sortKey="Qadri, S K" uniqKey="Qadri S">S.K. Qadri</name></author><author><name sortKey="Muraduzzaman, A K" uniqKey="Muraduzzaman A">A.K. 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Shehata</name><name sortKey="Teubner, Lisa" sort="Teubner, Lisa" uniqKey="Teubner L" first="Lisa" last="Teubner">Lisa Teubner</name></noCountry><country name="Liban"><noRegion><name sortKey="Kayali, Ghazi" sort="Kayali, Ghazi" uniqKey="Kayali G" first="Ghazi" last="Kayali">Ghazi Kayali</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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